dSpCas9-VPR
(Plasmid
#205247)
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PurposeIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneIVTpT7
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedSpCas9-VPR
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert is derived from Addgene plasmid #63798
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dSpCas9-VPR was a gift from Rasmus Bak (Addgene plasmid # 205247 ; http://n2t.net/addgene:205247 ; RRID:Addgene_205247) -
For your References section:
Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi. Jensen TI, Mikkelsen NS, Gao Z, Fosselteder J, Pabst G, Axelgaard E, Laustsen A, Konig S, Reinisch A, Bak RO. Genome Res. 2021 Nov;31(11):2120-2130. doi: 10.1101/gr.275607.121. Epub 2021 Aug 18. 10.1101/gr.275607.121 PubMed 34407984
Map uploaded by the depositor.
