ePPE-V223A
(Plasmid
#205243)
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PurposeFor plant prime editing in wheat plants or monocotyledons protoplasts
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205243 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJIT163
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSV40_NLS-nCas9-NC-MLV-SV40_NLS
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Alt namenCas9-NC-MLV
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SpeciesSynthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
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Insert Size (bp)6048
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MutationDetailed in manuscript
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer UBI-F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ePPE-V223A was a gift from Zong Yuan (Addgene plasmid # 205243 ; http://n2t.net/addgene:205243 ; RRID:Addgene_205243) -
For your References section:
Efficient and versatile multiplex prime editing in hexaploid wheat. Ni P, Zhao Y, Zhou X, Liu Z, Huang Z, Ni Z, Sun Q, Zong Y. Genome Biol. 2023 Jun 29;24(1):156. doi: 10.1186/s13059-023-02990-1. 10.1186/s13059-023-02990-1 PubMed 37386475
Map uploaded by the depositor.
