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Addgene

pHR- flag-Lag16-JAM-B
(Plasmid #205200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synCAM JAM-B and Lag16 anti-GFP nanobody
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The GFP nanobodies are from the Rout lab. Please see: A robust pipeline for rapid production of versatile nanobody repertoires. Nature Methods. 2014;11:1253–1260.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR- flag-Lag16-JAM-B was a gift from Wendell Lim (Addgene plasmid # 205200 ; http://n2t.net/addgene:205200 ; RRID:Addgene_205200)

  • For your References section:

    Programming multicellular assembly with synthetic cell adhesion molecules. Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA. Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z. Epub 2022 Dec 12. 10.1038/s41586-022-05622-z PubMed 36509107
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