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pGEMHE-RnCENP-O-11NS-GFP
(Plasmid #205181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEMHE
  • Backbone size w/o insert (bp) 3043
  • Total vector size (bp) 4675
  • Vector type
    Mammalian Expression ; In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-O
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    1632
  • Mutation
    Rat CENP-O with 11 mouse-specific point mutations in rapidly evolving residues (V99, C123, V141, R177, W179, A186, R225, C226, T228, N247, P261)
  • GenBank ID
    NC_000078.7 (mouse) NC_051341.1 (rat)
  • Entrez Gene
    Cenpo (a.k.a. 2810429O05Rik, 8430427C03Rik, D12Ertd482e)
  • Entrez Gene
    Cenpo
  • Promoter T7
  • Tag / Fusion Protein
    • 5 glycines linker followed by GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMHE-RnCENP-O-11NS-GFP was a gift from Michael Lampson (Addgene plasmid # 205181 ; http://n2t.net/addgene:205181 ; RRID:Addgene_205181)

  • For your References section:

    FREEDA: An automated computational pipeline guides experimental testing of protein innovation. Dudka D, Akins RB, Lampson MA. J Cell Biol. 2023 Sep 4;222(9):e202212084. doi: 10.1083/jcb.202212084. Epub 2023 Jun 26. 10.1083/jcb.202212084 PubMed 37358475
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