pRLuc-IVT
(Plasmid
#205148)
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PurposeIVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcGFP-C1
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Vector typeLuciferase ; In-vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRenilla Luciferase
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GenBank IDM15077
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer GTGTACGGTGGGAGGTCTAT
- 3′ sequencing primer GGTGGATCCCGGGCCCGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRLuc-IVT was a gift from Igor Ulitsky (Addgene plasmid # 205148 ; http://n2t.net/addgene:205148 ; RRID:Addgene_205148)