pYPQ141B-ge4.1
(Plasmid
#205144)
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PurposeGuide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYPQ104
- Total vector size (bp) 3363
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA cloning site
- Promoter AtU3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (unknown if destroyed)
- 3′ cloning site BsmBI (unknown if destroyed)
- 5′ sequencing primer M13-F
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We observed 1 base pair mismatch between Addgene's sequence and the depositor's sequence, present in the ge4.1_5' region. It does not affect the plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ141B-ge4.1 was a gift from Yiping Qi (Addgene plasmid # 205144 ; http://n2t.net/addgene:205144 ; RRID:Addgene_205144) -
For your References section:
Genome editing in rice and tomato with a small Un1Cas12f1 nuclease. Tang X, Eid A, Zhang R, Cheng Y, Liu A, Chen Y, Chen P, Zhang Y, Qi Y. Plant Genome. 2024 May 28:e20465. doi: 10.1002/tpg2.20465. 10.1002/tpg2.20465 PubMed 38807445
