pYPQ-Un1Cas12f1
(Plasmid
#205143)
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PurposeUn1Cas12f1 Gateway entry plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYPQ167
- Total vector size (bp) 4842
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUn1Cas12f1
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Tags
/ Fusion Proteins
- NLS (C terminal on insert)
- NLS (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ-Un1Cas12f1 was a gift from Yiping Qi (Addgene plasmid # 205143 ; http://n2t.net/addgene:205143 ; RRID:Addgene_205143) -
For your References section:
Genome editing in rice and tomato with a small Un1Cas12f1 nuclease. Tang X, Eid A, Zhang R, Cheng Y, Liu A, Chen Y, Chen P, Zhang Y, Qi Y. Plant Genome. 2024 May 28:e20465. doi: 10.1002/tpg2.20465. 10.1002/tpg2.20465 PubMed 38807445
