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Addgene

pJV0047
(Plasmid #205139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOCC10
  • Backbone size w/o insert (bp) 5976
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tandem 2xPX domain of SnxA
  • Alt name
    DDB_G0289833
  • Species
    Synthetic
  • Insert Size (bp)
    851
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJV0047 was a gift from Jason King (Addgene plasmid # 205139 ; http://n2t.net/addgene:205139 ; RRID:Addgene_205139)

  • For your References section:

    A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes. Vines JH, Maib H, Buckley CM, Gueho A, Zhu Z, Soldati T, Murray DH, King JS. J Cell Biol. 2023 Sep 4;222(9):e202209077. doi: 10.1083/jcb.202209077. Epub 2023 Jun 29. 10.1083/jcb.202209077 PubMed 37382666
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