pCB128
(Plasmid #205137)

• Purpose: Expression of mCherry-2xFYVE in Dictyostelium
• Depositing Lab: Jason King
• Publication: Vines et al J Cell Biol. 2023 Sep 4;222(9):e202209077. doi: 10.1083/jcb.202209077. Epub 2023 Jun 29.

Backbone

• Vector backbone: pDM1208
• Backbone size w/o insert (bp): 7638
• Vector type: Dictyostelium expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2xFYVE (from human Hrs)
• Species: Synthetic
• Insert Size (bp): 519
• Entrez Gene: HGS (a.k.a. HRS)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pCB128.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: We have identified several potential errors in the highly non-complex and repetitive published sequence of the Dictyostelium pDM vector backbone. These are all in non-coding highly repetitive regions so likely not important for function.