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pJV0016
(Plasmid #205133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJSK1045
  • Backbone size w/o insert (bp) 7964
  • Vector type
    Dictyostelium expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tandem 2xPX domain of SnxA
  • Alt name
    DDB_G0289833
  • Species
    Dictyostelium discoideum
  • Insert Size (bp)
    812
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: We have identified several potential errors in the highly non-complex and repetitive published sequence of the Dictyostelium pDM vector backbone. These are all in non-coding highly repetitive regions so likely not important for function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJV0016 was a gift from Jason King (Addgene plasmid # 205133 ; http://n2t.net/addgene:205133 ; RRID:Addgene_205133)

  • For your References section:

    A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes. Vines JH, Maib H, Buckley CM, Gueho A, Zhu Z, Soldati T, Murray DH, King JS. J Cell Biol. 2023 Sep 4;222(9):e202209077. doi: 10.1083/jcb.202209077. Epub 2023 Jun 29. 10.1083/jcb.202209077 PubMed 37382666
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