LRP1A-c004
(Plasmid
#205112)
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PurposeLRP1 protein expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFB-Sec-Bio5
- Backbone size w/o insert (bp) 6615
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLRP1
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Alt nameA2MR
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Alt nameAPR
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SpeciesH. sapiens (human)
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MutationWT
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Entrez GeneLRP1 (a.k.a. A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R, IGFBP3R1, KPA, LRP, LRP1A, TGFBR5)
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Tags
/ Fusion Proteins
- GP64sp-6His-TEV (N terminal on insert)
- AviTag (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Encodes residues 20-172. Can be biotinylated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LRP1A-c004 was a gift from Paul Brennan (Addgene plasmid # 205112 ; http://n2t.net/addgene:205112 ; RRID:Addgene_205112)