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Addgene

CAPN2A-c002
(Plasmid #205104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone size w/o insert (bp) 5289
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAPN2
  • Alt name
    CANPL2
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    CAPN2 (a.k.a. CANP2, CANPL2, CANPml, mCANP)
  • Tags / Fusion Proteins
    • 6His-TEV (N terminal on insert)
    • AviTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes full length CAPN2 and residues 86-268 of CAPNS1. CAPN2 can be biotinylated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAPN2A-c002 was a gift from Paul Brennan (Addgene plasmid # 205104 ; http://n2t.net/addgene:205104 ; RRID:Addgene_205104)
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