pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)
(Plasmid #205098)

• Purpose: Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogenetics
• Depositing Lab: Ute Hochgeschwender
• Publication: Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. doi: 10.1101/2023.06.26.545546. Preprint.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5313
• Total vector size (bp): 7517
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mNeonGreen
• Alt name: mNG
• Species: Synthetic
• Insert Size (bp): 678

Gene/Insert 2

• Gene/Insert name: eKaz luciferase
• Alt name: eKL9h
• Species: Synthetic
• Insert Size (bp): 501

Gene/Insert 3

• Gene/Insert name: Volvox Channelrhodopsin 1
• Alt name: VChR1
• Species: Synthetic
• Insert Size (bp): 895

Resource Information

• Supplemental Documents:
  • pAAV Ef1a DIO LMO7.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.26.545546 for bioRxiv preprint.