Zyxin-GFP
(Plasmid #205095)

• Purpose: Express GFP-tagged human zyxin in mammalian cells
• Depositing Lab: Bianxiao Cui
• Publication: Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038/s41556-023-01238-1.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6445
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: zyxin
• Species: H. sapiens (human)
• Insert Size (bp): 1723
• Entrez Gene: ZYX (a.k.a. ESP-2, HED-2)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: AGCTC AAGCTT cggccatggcggcc
• 3′ sequencing primer: GTA CCG TCG ACT GGG TCT GGG CTC TAG CAG TGT G

Resource Information

• Supplemental Documents:
  • Zyxin-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry