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Addgene

pV1F
(Plasmid #205084)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205084 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p302T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AtVIP1
  • Alt name
    arabidopsis VirE2-interacting Protein 1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1023
  • GenBank ID
    NC_003070.9
  • Promoter agorbacterium virB operon promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CGGGGTACCATGCCTGTCGAGTCGGCTGAGAATATA
  • 3′ sequencing primer CCGGAATTCTCATAGACCGCGCGTTGATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pV1F was a gift from Suomeng Dong (Addgene plasmid # 205084 ; http://n2t.net/addgene:205084 ; RRID:Addgene_205084)

  • For your References section:

    A modified Agrobacterium-mediated transformation for two oomycete pathogens. Wang L, Zhao F, Liu H, Chen H, Zhang F, Li S, Sun T, Nekrasov V, Huang S, Dong S. PLoS Pathog. 2023 Apr 21;19(4):e1011346. doi: 10.1371/journal.ppat.1011346. eCollection 2023 Apr. 10.1371/journal.ppat.1011346 PubMed 37083862
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