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Addgene

T7RNAP Cyslight V174C
(Plasmid #205078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T7RNAP Cyslight V174C
  • Species
    Bacteriophage T7
  • Mutation
    C125S, C347S, C510S, C515S, C530S, C723S V174C
  • Entrez Gene
    T7p07 (a.k.a. T7p07)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7RNAP Cyslight V174C was a gift from Smita Patel (Addgene plasmid # 205078 ; http://n2t.net/addgene:205078 ; RRID:Addgene_205078)

  • For your References section:

    Correlating Transcription Initiation and Conformational Changes by a Single-Subunit RNA Polymerase with Near Base-Pair Resolution. Koh HR, Roy R, Sorokina M, Tang GQ, Nandakumar D, Patel SS, Ha T. Mol Cell. 2018 May 17;70(4):695-706.e5. doi: 10.1016/j.molcel.2018.04.018. 10.1016/j.molcel.2018.04.018 PubMed 29775583
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