MTF1 deltaC20
(Plasmid
#205071)
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PurposeMTF1 protein expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMTF1
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SpeciesS. cerevisiae (budding yeast)
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MutationLacks C-terminal 20 amino acids
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Entrez GeneMTF1 (a.k.a. YMR228W)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MTF1 deltaC20 was a gift from Smita Patel (Addgene plasmid # 205071 ; http://n2t.net/addgene:205071 ; RRID:Addgene_205071) -
For your References section:
The C-terminal tail of the yeast mitochondrial transcription factor Mtf1 coordinates template strand alignment, DNA scrunching and timely transition into elongation. Basu U, Lee SW, Deshpande A, Shen J, Sohn BK, Cho H, Kim H, Patel SS. Nucleic Acids Res. 2020 Mar 18;48(5):2604-2620. doi: 10.1093/nar/gkaa040. 10.1093/nar/gkaa040 PubMed 31980825