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Addgene

mt POLRMT
(Plasmid #205059)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205059 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    POLRMT
  • Species
    H. sapiens (human)
  • Mutation
    Codon optimized
  • Entrez Gene
    POLRMT (a.k.a. APOLMT, COXPD55, MTRNAP, MTRPOL, h-mtRPOL)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mt POLRMT was a gift from Smita Patel (Addgene plasmid # 205059 ; http://n2t.net/addgene:205059 ; RRID:Addgene_205059)
  • For your References section:

    Human mitochondrial transcription factors TFAM and TFB2M work synergistically in promoter melting during transcription initiation. Ramachandran A, Basu U, Sultana S, Nandakumar D, Patel SS. Nucleic Acids Res. 2017 Jan 25;45(2):861-874. doi: 10.1093/nar/gkw1157. Epub 2016 Nov 29. 10.1093/nar/gkw1157 PubMed 27903899