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Addgene

SUMO-Twinkle 360-684
(Plasmid #205055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TWNK
  • Species
    H. sapiens (human)
  • Mutation
    Lacks N terminal amino acid from 1-359
  • Entrez Gene
    TWNK (a.k.a. ATXN8, C10orf2, IOSCA, MTDPS7, PEO, PEO1, PEOA3, PRLTS5, SANDO, SCA8, TWINL)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SUMO-Twinkle 360-684 was a gift from Smita Patel (Addgene plasmid # 205055 ; http://n2t.net/addgene:205055 ; RRID:Addgene_205055)

  • For your References section:

    The N-terminal domain of human mitochondrial helicase Twinkle has DNA-binding activity crucial for supporting processive DNA synthesis by polymerase gamma. Johnson LC, Singh A, Patel SS. J Biol Chem. 2023 Jan;299(1):102797. doi: 10.1016/j.jbc.2022.102797. Epub 2022 Dec 14. 10.1016/j.jbc.2022.102797 PubMed 36528058
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