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Addgene

pET-6xHis-GFP(0)-L1
(Plasmid #205033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP(0)-L1
  • Alt name
    GFP(0)-(GRRGKKSRK)1
  • Species
    Other
  • Mutation
    E16R, I138R, D207R, N222K, L246R
  • Tags / Fusion Proteins
    • MGHHHHHGGA (N terminal on insert)
    • GRRGKKSRK (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-6xHis-GFP(0)-L1 was a gift from Allie Obermeyer (Addgene plasmid # 205033 ; http://n2t.net/addgene:205033 ; RRID:Addgene_205033)

  • For your References section:

    Charge-Patterned Disordered Peptides Tune Intracellular Phase Separation in Bacteria. Liao J, Yeong V, Obermeyer AC. ACS Synth Biol. 2024 Feb 3. doi: 10.1021/acssynbio.3c00564. 10.1021/acssynbio.3c00564 PubMed 38308651
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