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E. coli BW25113 ΔtnaA ΔtrpR
(Bacterial strain #205015)

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Item Catalog # Description Quantity Price (USD)
Bacterial Strain 205015 Bacteria in agar stab 1 $85 *
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli BW25113
  • Growth instructions
    This strain can be cultivated in liquid LB medium without antibiotics under 37 ℃ and harvested after 12 h.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ΔtnaA ΔtrpR
  • Species
    E. coli

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Primers for PCR verification:
veri-trpR-F: AGCAGCTTATAACGCCGGACCAGGG
veri-trpR-R: TGGTCCCGTGATGTCGCGTTATAC
veri-tnaA-F: CTTGTTTTAGTAAATGATGGTGCTTG
veri-tnaA-R: GATCAGTCATGATGCCACCTTTAGAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli BW25113 ΔtnaA ΔtrpR was a gift from Yajun Yan (Addgene plasmid # 205015)

  • For your References section:

    Engineering of a TrpR-Based Biosensor for Altered Dynamic Range and Ligand Preference. Gong X, Zhang R, Wang J, Yan Y. ACS Synth Biol. 2022 Jun 17;11(6):2175-2183. doi: 10.1021/acssynbio.2c00134. Epub 2022 May 20. 10.1021/acssynbio.2c00134 PubMed 35594503
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