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Addgene

pHeEf1a-mEGFP-NLS
(Plasmid #205012)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205012 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 2021
  • Vector type
    Tardigrade expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
  • Species
    Hypsibius exemplaris, Tardigrade
  • Insert Size (bp)
    1000

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHeEf1a-mEGFP-NLS was a gift from Kazuharu Arakawa (Addgene plasmid # 205012 ; http://n2t.net/addgene:205012 ; RRID:Addgene_205012)

  • For your References section:

    In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada. Tanaka S, Aoki K, Arakawa K. Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2216739120. doi: 10.1073/pnas.2216739120. Epub 2023 Jan 24. 10.1073/pnas.2216739120 PubMed 36693101
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