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Addgene

pET28a_CnCas12f1
(Plasmid #204996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CnCas12f1
  • Species
    Synthetic
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_CnCas12f1 was a gift from Quanjiang Ji (Addgene plasmid # 204996 ; http://n2t.net/addgene:204996 ; RRID:Addgene_204996)

  • For your References section:

    Molecular basis and engineering of miniature Cas12f with C-rich PAM specificity. Su M, Li F, Wang Y, Gao Y, Lan W, Shao Z, Zhu C, Tang N, Gan J, Wu Z, Ji Q. Nat Chem Biol. 2024 Feb;20(2):180-189. doi: 10.1038/s41589-023-01420-4. Epub 2023 Sep 11. 10.1038/s41589-023-01420-4 PubMed 37697004
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