pMB3-A-TelR
(Plasmid
#204991)
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PurposeLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTU3-A-RFP / pSB1A2
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Backbone manufacturerPaul Freemont Lab / iGem (Addgene plasmid # 72945)
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Vector typeBacterial Expression, Synthetic Biology
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Selectable markersmRFP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUse Tellurite for selection in A. baumannii
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP1
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SpeciesSynthetic
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MutationAdded replications elements for Acinetobacter species into the PstI site and Tellurite resistance cassette into XbaI site
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byOriginal base plasmid was received from Addgene, Tellurite resistance cassette from pMO130-TelR (50799)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is related to, but is not included with, the AspFlex Kit (Addgene #1000000217: https://www.addgene.org/kits/godoy-carter-aspflex/)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMB3-A-TelR was a gift from Veronica Godoy-Carter (Addgene plasmid # 204991 ; http://n2t.net/addgene:204991 ; RRID:Addgene_204991) -
For your References section:
AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii. Brychcy M, Kokodynski A, Lloyd D, Godoy VG. ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. 10.1021/acssynbio.3c00167 PubMed 37587063