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Addgene

pMB2-D-TelR
(Plasmid #204990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTU2-D-RFP / pSB1C3
  • Backbone manufacturer
    Paul Freemont Lab / iGem (Addgene plasmid # 72957)
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    mRFP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use Tellurite for selection in A. baumannii
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP1
  • Species
    Synthetic
  • Mutation
    Added replications elements for Acinetobacter species into the PstI site and Tellurite resistance cassette into XbaI site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Original base plasmid was received from Addgene, Tellurite resistance cassette from pMO130-TelR (50799)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is related to, but is not included with, the AspFlex Kit (Addgene #1000000217: https://www.addgene.org/kits/godoy-carter-aspflex/)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB2-D-TelR was a gift from Veronica Godoy-Carter (Addgene plasmid # 204990 ; http://n2t.net/addgene:204990 ; RRID:Addgene_204990)

  • For your References section:

    AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii. Brychcy M, Kokodynski A, Lloyd D, Godoy VG. ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. 10.1021/acssynbio.3c00167 PubMed 37587063
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