pMB1-A-TelR
(Plasmid #204979)

• Purpose: Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
• Depositing Lab: Veronica Godoy-Carter
• Publication: Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.

Backbone

• Vector backbone: pTU1-A-RFP / pSB1A2
• Backbone manufacturer: Paul Freemont Lab / iGem (Addgene plasmid # 72939)
• Vector type: Bacterial Expression, Synthetic Biology
• Selectable markers: mRFP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use Tellurite for selection in A. baumannii
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRFP1
• Species: Synthetic
• Mutation: Added replications elements for Acinetobacter species into the PstI site and Tellurite resistance cassette into XbaI site

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pMB1-A-TelR Genbank
• A portion of this plasmid was derived from a plasmid made by: Original base plasmid was received from Addgene, Tellurite resistance cassette from pMO130-TelR (50799)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is related to, but is not included with, the AspFlex Kit (Addgene #1000000217: https://www.addgene.org/kits/godoy-carter-aspflex/)