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pCMV-AsCas12f1-v5.1+T1-empty
(Plasmid #204977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Col1E
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AsCas12f1-v5.1, sgRNA_T1
  • Alt name
    Cas12U3
  • Species
    Synthetic
  • Mutation
    N70Q/K103R/A104R/S118A/D364R
  • Promoter PCMV,U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-AsCas12f1-v5.1+T1-empty was a gift from Quanjiang Ji (Addgene plasmid # 204977 ; http://n2t.net/addgene:204977 ; RRID:Addgene_204977)

  • For your References section:

    Structure and engineering of miniature Acidibacillus sulfuroxidans Cas12f1. Wu Z, Liu D, Pan D, Yu H, Shi J, Ma J, Fu W, Wang Z, Zheng Z, Qu Y, Li F, Chen W, Huang X, Shen H, Ji Q. Nature Catalysis (2023) 6, 695–709 10.1038/s41929-023-00995-4
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