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MsACR1-pmCerulean3-N1
(Plasmid #204958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 3987
  • Total vector size (bp) 5427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MsACR1
  • Species
    Mantoniella squamata
  • Insert Size (bp)
    723
  • Promoter CMV (+enhancer)
  • Tag / Fusion Protein
    • mCerulean3 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CMV-fwd: GCAAATGGGCGGTAGGCGT
  • 3′ sequencing primer EGFP-C1-rev: AACCATTATAAGCTGCAATAAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.09.544329 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MsACR1-pmCerulean3-N1 was a gift from Peter Hegemann (Addgene plasmid # 204958 ; http://n2t.net/addgene:204958 ; RRID:Addgene_204958)

  • For your References section:

    Robust Optogenetic Inhibition with Red-light-sensitive Anion-conducting Channelrhodopsins. Oppermann J, Rozenberg A, Fabrin T, GonzalezCabrera C, Béjà O, Prigge M, Hegemann P. eLife 2023 (Reviewed Preprint) 10.7554/eLife.90100.1
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