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pAAV-ITR-PytR(PyOtR)-mCherry
(Plasmid #204870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Cell Biolabs
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5849
  • Modifications to backbone
    Replaced GFP with mCherry fluorescent reporter
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M. mazei pyrrolysyl tRNA mutant "PyOtR"
  • Alt name
    PyOtR
  • Alt name
    HTS-25
  • Alt name
    PytR.PyOTR
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    69
  • Mutation
    U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer TTATTTACCTAGGGGTACCTCGGGCAGGAAGAGGGCCTATTTCCCATG
  • 3′ sequencing primer GATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-ITR-PytR(PyOtR)-mCherry was a gift from Abhishek Chatterjee (Addgene plasmid # 204870 ; http://n2t.net/addgene:204870 ; RRID:Addgene_204870)

  • For your References section:

    Enhanced Directed Evolution in Mammalian Cells Yields a Hyperefficient Pyrrolysyl tRNA for Noncanonical Amino Acid Mutagenesis. Jewel D, Kelemen RE, Huang RL, Zhu Z, Sundaresh B, Malley K, Pham Q, Loynd C, Huang Z, van Opijnen T, Chatterjee A. Angew Chem Int Ed Engl. 2024 Jan 26:e202316428. doi: 10.1002/anie.202316428. 10.1002/anie.202316428 PubMed 38279536
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