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pAAV-ITR-PytR-mCherry
(Plasmid #204868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Cell Biolabs
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5849
  • Modifications to backbone
    Replaced GFP with mCherry fluorescent reporter
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M. mazei pyrrolysyl tRNA
  • Alt name
    PylT
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    69
  • Mutation
    U25C
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer TTATTTACCTAGGGGTACCTCGGGCAGGAAGAGGGCCTATTTCCCATG
  • 3′ sequencing primer GATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-ITR-PytR-mCherry was a gift from Abhishek Chatterjee (Addgene plasmid # 204868 ; http://n2t.net/addgene:204868 ; RRID:Addgene_204868)

  • For your References section:

    Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells. Jewel D, Kelemen RE, Huang RL, Zhu Z, Sundaresh B, Cao X, Malley K, Huang Z, Pasha M, Anthony J, van Opijnen T, Chatterjee A. Nat Methods. 2023 Jan;20(1):95-103. doi: 10.1038/s41592-022-01706-w. Epub 2022 Dec 22. 10.1038/s41592-022-01706-w PubMed 36550276
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