MPRA_minCMV+GFP
(Plasmid #204866)

• Purpose: Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.
• Depositing Lab: Philipp Maass
• Publication: Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi: 10.1016/j.xgen.2023.100330. eCollection 2023 Jul 12.

Backbone

• Vector backbone: pGL4.23
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2642
• Total vector size (bp): 3481
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: minCMV:GFP
• Alt name: peTurboGFP-dest1
• Species: Pontellina plumata
• Insert Size (bp): 839
• Promoter: minimal CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CGCCGCGTGGAGGAGGA
• 3′ sequencing primer: CGACGCTCTTCCGATCT

Resource Information

• Supplemental Documents:
  • MPRA_minCMV_GFP.gb
• A portion of this plasmid was derived from a plasmid made by: Rinn Lab, CU Boulder

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry