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pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2
(Plasmid #204852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
  • Backbone manufacturer
    Feng Zhang, Addgene #61591
  • Vector type
    AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniSdd6-nSaCas9KKH(D10A)-UGI
  • Alt name
    mouse HPD sgRNA
  • Species
    Synthetic
  • Mutation
    D10A, E782K, N968K, R1015H in SaCas9
  • Promoter EFS, miniU6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.21.541555 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2 was a gift from Caixia Gao (Addgene plasmid # 204852 ; http://n2t.net/addgene:204852 ; RRID:Addgene_204852)

  • For your References section:

    Discovery of deaminase functions by structure-based protein clustering. Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT, Gao C. Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 10.1016/j.cell.2023.05.041. Epub 2023 Jun 27. 10.1016/j.cell.2023.05.041 PubMed 37379837
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