pLenti-hSynapsin-Nluc-EPAC-VV
(Plasmid
#204846)
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PurposeProduce lentivirus encoding Nluc-EPAC sensor in neuronal cell type
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-hSynapsin
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNluc-EPAC sensor
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-hSynapsin-Nluc-EPAC-VV was a gift from Mikel Garcia-Marcos (Addgene plasmid # 204846 ; http://n2t.net/addgene:204846 ; RRID:Addgene_204846) -
For your References section:
Fine-tuning GPCR-mediated neuromodulation by biasing signaling through different G protein subunits. Park JC, Luebbers A, Dao M, Semeano A, Nguyen AM, Papakonstantinou MP, Broselid S, Yano H, Martemyanov KA, Garcia-Marcos M. Mol Cell. 2023 Jul 20;83(14):2540-2558.e12. doi: 10.1016/j.molcel.2023.06.006. Epub 2023 Jun 29. 10.1016/j.molcel.2023.06.006 PubMed 37390816