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Addgene

pLIC-GST-GINIP
(Plasmid #204844)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204844 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLIC-GST
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHF24
  • Alt name
    KIAA1045
  • Alt name
    GINIP
  • Species
    H. sapiens (human)
  • Entrez Gene
    KIAA1045 (a.k.a. DKFZp434I2112, RP11-392A14.4)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GINIP sequence was modified for codon optimization for bacterial expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC-GST-GINIP was a gift from Mikel Garcia-Marcos (Addgene plasmid # 204844 ; http://n2t.net/addgene:204844 ; RRID:Addgene_204844)
  • For your References section:

    Fine-tuning GPCR-mediated neuromodulation by biasing signaling through different G protein subunits. Park JC, Luebbers A, Dao M, Semeano A, Nguyen AM, Papakonstantinou MP, Broselid S, Yano H, Martemyanov KA, Garcia-Marcos M. Mol Cell. 2023 Jul 20;83(14):2540-2558.e12. doi: 10.1016/j.molcel.2023.06.006. Epub 2023 Jun 29. 10.1016/j.molcel.2023.06.006 PubMed 37390816