pLIC-GST-GINIP
(Plasmid
#204844)
-
PurposeExpress GST tagged-GINIP in bacterial cells for protein purification
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 204844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLIC-GST
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePHF24
-
Alt nameKIAA1045
-
Alt nameGINIP
-
SpeciesH. sapiens (human)
-
Entrez GeneKIAA1045 (a.k.a. DKFZp434I2112, RP11-392A14.4)
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GINIP sequence was modified for codon optimization for bacterial expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLIC-GST-GINIP was a gift from Mikel Garcia-Marcos (Addgene plasmid # 204844 ; http://n2t.net/addgene:204844 ; RRID:Addgene_204844) -
For your References section:
Fine-tuning GPCR-mediated neuromodulation by biasing signaling through different G protein subunits. Park JC, Luebbers A, Dao M, Semeano A, Nguyen AM, Papakonstantinou MP, Broselid S, Yano H, Martemyanov KA, Garcia-Marcos M. Mol Cell. 2023 Jul 20;83(14):2540-2558.e12. doi: 10.1016/j.molcel.2023.06.006. Epub 2023 Jun 29. 10.1016/j.molcel.2023.06.006 PubMed 37390816