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Addgene

pRK5 BMPS HK318/9EE-6xHis
(Plasmid #204836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLN5
  • Alt name
    BMPS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Mutation
    HK318/9EE
  • Entrez Gene
    CLN5
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer TCCCAGGTCCAACTGCACCTCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 BMPS HK318/9EE-6xHis was a gift from Monther Abu-Remaileh (Addgene plasmid # 204836 ; http://n2t.net/addgene:204836 ; RRID:Addgene_204836)

  • For your References section:

    The Batten disease gene product CLN5 is the lysosomal bis(monoacylglycero)phosphate synthase. Medoh UN, Hims A, Chen JY, Ghoochani A, Nyame K, Dong W, Abu-Remaileh M. Science. 2023 Sep 15;381(6663):1182-1189. doi: 10.1126/science.adg9288. 10.1126/science.adg9288 PubMed 37708259
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