pDB5321
(Plasmid #204831)

• Purpose: An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
• Depositing Lab: Li-Lin Du
• Publication: Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404. doi: 10.1242/jcs.263404. Epub 2024 Nov 1.

Backbone

• Vector backbone: pAde6NotI
• Backbone size w/o insert (bp): 8615
• Total vector size (bp): 9950
• Vector type: Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: tetR
• Species: S. pombe (fission yeast)
• Insert Size (bp): 621
• Promoter: CMV promoter

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: tgcaaggcgattaagttg

Gene/Insert 2

• Gene/Insert name: mECitrine
• Species: S. pombe (fission yeast)
• Insert Size (bp): 714
• Promoter: enotetSW3 promoter

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: TCAACATGGCGGTAATGT

Resource Information

• Supplemental Documents:
  • Protocol for the improved tetracycline-inducible expression system
  • Depositor Annotated Sequence pDB5321-#204831.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry