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pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP
(Plasmid #204821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Backbone size w/o insert (bp) 8116
  • Total vector size (bp) 8855
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    An upstream region including human SCGB3A2 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    739
  • GenBank ID
    NM_054023
  • Promoter SCGB3A2 promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATTGAATCCCAGGTTTTTCAAAAGACACT
  • 3′ sequencing primer GACAGTTATCTGGGATATTTTTCAGGAGTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP was a gift from Emma Rawlins (Addgene plasmid # 204821 ; http://n2t.net/addgene:204821 ; RRID:Addgene_204821)

  • For your References section:

    A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates. He P, Lim K, Sun D, Pett JP, Jeng Q, Polanski K, Dong Z, Bolt L, Richardson L, Mamanova L, Dabrowska M, Wilbrey-Clark A, Madissoon E, Tuong ZK, Dann E, Suo C, Goh I, Yoshida M, Nikolic MZ, Janes SM, He X, Barker RA, Teichmann SA, Marioni JC, Meyer KB, Rawlins EL. Cell. 2022 Dec 8;185(25):4841-4860.e25. doi: 10.1016/j.cell.2022.11.005. 10.1016/j.cell.2022.11.005 PubMed 36493756
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