DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3
(Plasmid
#204808)
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PurposeBacterial co-expression of Dengue2 NS2B and NS3 proteins
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepNIC-6HIS-SUMO-GG
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDengue2 NS2B
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SpeciesDengue2
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Entrez GenePOLY (a.k.a. DENV_gp1, polyprotein gene)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3 was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 204808 ; http://n2t.net/addgene:204808 ; RRID:Addgene_204808)
