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Addgene

WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein
(Plasmid #204794)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 204794 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pNIC-NHStIIT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    West Nile NS2B-NS3 fusion
  • Species
    West Nile

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 204794 ; http://n2t.net/addgene:204794 ; RRID:Addgene_204794)