MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL
(Plasmid
#204790)
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PurposeBacterial expression of MERS Mpro
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepNIC-6HIS-SUMO-GG
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMERS Mpro
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SpeciesMERS
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MutationMSAVMQ/SGLVKM
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 204790 ; http://n2t.net/addgene:204790 ; RRID:Addgene_204790)