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Addgene

SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like
(Plasmid #204777)

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Item Catalog # Description Quantity Price (USD)
Plasmid 204777 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-LIC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sars Cov 2 MPRO
  • Species
    Sars Cov 2
  • Mutation
    M49I
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 204777 ; http://n2t.net/addgene:204777 ; RRID:Addgene_204777)
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