SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like
(Plasmid
#204769)
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PurposeBacterial expression of Sars Cov 2 MPRO C145S mutant
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 204769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-LIC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSars Cov 2 MPRO
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SpeciesSars Cov 2
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MutationC145S mutant
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
Cloning Information
- Cloning method Unknown
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 204769 ; http://n2t.net/addgene:204769 ; RRID:Addgene_204769)