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Addgene

pCPH2A.X_S139D-GS3
(Plasmid #204748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-Puro(PX459)V2.0(#62988)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2AX S139D
  • Alt name
    H2AFX
  • Species
    H. sapiens (human)
  • Mutation
    H2AX with the S139D mutation
  • Entrez Gene
    H2AX (a.k.a. H2A.X, H2A/X, H2AFX)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCPH2A.X_S139D-GS3 was a gift from Yuichiro Miyaoka (Addgene plasmid # 204748 ; http://n2t.net/addgene:204748 ; RRID:Addgene_204748)

  • For your References section:

    Fusion of histone variants to Cas9 suppresses non-homologous end joining. Kato-Inui T, Takahashi G, Ono T, Miyaoka Y. PLoS One. 2024 May 13;19(5):e0288578. doi: 10.1371/journal.pone.0288578. eCollection 2024. 10.1371/journal.pone.0288578 PubMed 38739603
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