pGB
(Plasmid
#204744)
-
Purpose(Empty Backbone) gRNA-Bsd expression vector
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 204744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSpCas9(BB)-2A-Puro(PX459)V2.0(#62988)
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGB was a gift from Yuichiro Miyaoka (Addgene plasmid # 204744 ; http://n2t.net/addgene:204744 ; RRID:Addgene_204744) -
For your References section:
Fusion of histone variants to Cas9 suppresses non-homologous end joining. Kato-Inui T, Takahashi G, Ono T, Miyaoka Y. PLoS One. 2024 May 13;19(5):e0288578. doi: 10.1371/journal.pone.0288578. eCollection 2024. 10.1371/journal.pone.0288578 PubMed 38739603