iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

Analyze Sequence

GenBank

SnapGene

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> Addgene NGS Result GGCGCGCCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCA TATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCC CATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGT GGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATT GACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTT GGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCT CCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATG GGGGCGGGGGGGGGGGGGGGGGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCG GAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGG CGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCC GCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCG GGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTG CGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGT GTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGC GGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGG GGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGT CGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGC GGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCT GTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTT GTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAG CGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTC TCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTT CGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTA CAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATGTCGACATAACTTCG TATAATGTATACTATACGAAGTTATTCGCGATGAATAAATGAAAGCTTGCAGATCTGCGACCCTAGAGGA TCTGCGACCCTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTC CCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTT ATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAG TTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGCGACCCTAGAGGATCATAATCA GCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACA TAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGC ATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATG TATCTTATCATGTCTGGATCTGCGACCCTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTA CTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACTAAAAATGAATGCAATTGTTGTTGTTA ACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATT TTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTCCATC AAGCTGATCCGGAACCCTTAATATAACTTCGTATAATGTATACTATACGAAGTTATGGTACCTCGCGAGC CGCCACCATGAACAACTCTCAGGGCAGAGTGACCTTCGAGGACGTGACCGTGAACTTCACCCAAGGCGAG TGGCAGAGACTGAACCCCGAGCAGAGAAACCTGTACCGGGACGTGATGCTGGAAAACTACAGCAACCTGG TGTCCGTCGGCCAGGGCGAGACAACAAAGCCTGACGTGATCCTGCGGCTGGAACAGGGCAAAGAGCCTTG GCTGGAAGAGGAAGAGGTGCTCGGATCTGGCAGAGCCGAGAAGAATGGCGACATCGGCGGACAGATCTGG AAGCCCAAGGACGTGAAAGAGTCTCTGAGTGGCGGCTCAAGTGGCGGGTCTAGTGGGAGTGAAACGCCAG GGACTTCAGAATCAGCAACCCCGGAATCATCCGGCGGATCATCCGGTGGGTCTGTATACATGGACAAGAA GTACTCTATCGGCCTGGCCATCGGCACCAATTCTGTTGGATGGGCCGTGATCACCGACGAGTACAAGGTG CCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAATCTGATCGGCGCCC TGCTGTTCGACTCTGGCGAAACAGCCGAAGCCACCAGACTGAAGAGAACCGCCAGACGGCGGTACACCAG AAGAAAGAACCGGATCTGCTACCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTC TTCCACAGACTGGAAGAGTCCTTCCTGGTCGAGGAAGATAAGAAGCACGAGCGGCACCCCATCTTCGGCA ACATCGTGGATGAGGTGGCCTACCACGAGAAGTACCCTACCATCTACCACCTCCGGAAGAAACTGGTGGA CAGCACCGACAAGGCCGACCTGAGACTGATCTATCTGGCCCTGGCTCACATGATCAAGTTCCGGGGCCAC TTTCTGATCGAGGGCGACCTGAATCCTGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGA CCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGAGTGGATGCCAAGGCCATCCTGTCTGC CAGACTGAGCAAGAGCAGACGCCTGGAAAACCTGATCGCTCAGCTGCCCGGCGAGAAGAAGAACGGCCTG TTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACACCTAACTTCAAGAGCAACTTCGACCTGGCTGAGG ACGCCAAACTGCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAATCTGCTGGCCCAGATCGGCGA TCAGTACGCCGACTTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGA GTGAACACCGAGATCACAAAGGCCCCTCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGG ATCTGACCCTGCTGAAGGCCCTCGTTAGACAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCA GAGCAAGAACGGCTACGCCGGCTACATTGATGGCGGAGCCAGCCAAGAGGAATTTTACAAGTTCATCAAG CCCATCCTTGAGAAGATGGACGGCACCGAGGAACTGCTGGTCAAGCTGAACAGAGAGGACCTGCTGCGGA AGCAGCGGACCTTCGACAATGGCTCTATCCCTCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCG GAGACAAGAGGACTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATTGAGAAGATCCTGACCTTCAGA ATCCCCTACTACGTGGGACCACTGGCCAGAGGCAATAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGG AAACCATCACACCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCCAGCGCTCAGTCCTTCATCGAGCG GATGACCAACTTCGATAAGAACCTGCCTAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTAC TTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTTCTGA GCGGCGAGCAGAAAAAGGCCATTGTGGATCTGCTGTTCAAGACCAACCGGAAAGTGACAGTGAAGCAGCT GAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACAGCGTGGAAATCAGCGGCGTGGAAGATCGGTTC AATGCCAGCCTGGGCACATACCACGACCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAACGAAG AGAACGAGGACATCCTGGAAGACATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGA ACGGCTGAAAACATACGCCCACCTGTTCGACGACAAAGTGATGAAGCAACTGAAGCGGCGGAGATACACC GGCTGGGGTAGACTGTCCCGGAAGCTGATCAACGGCATCCGGGATAAGCAGTCCGGCAAGACCATCCTGG ACTTTCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTT TAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAAGGCGATTCTCTGCACGAGCACATTGCCAACCTG GCCGGATCTCCCGCCATTAAGAAGGGCATCCTGCAGACCGTGAAGGTGGTGGACGAGCTTGTGAAAGTGA TGGGCAGACACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACACAGAAGGGCCA GAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAA GAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGACGGG ATATGTACGTGGACCAAGAGCTGGACATCAACCGGCTGTCCGACTACGATGTGGACGCTATCGTGCCCCA GTCTTTTCTGAAGGACGACTCCATCGACAACAAGGTCCTGACCAGAAGCGACAAGAACCGGGGCAAGAGC GATAACGTGCCCTCCGAAGAGGTGGTCAAGAAGATGAAGAACTATTGGCGCCAGCTGCTGAATGCCAAGC TGATTACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGAAAGAGGCGGCCTGAGCGAACTGGATAAGGC CGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACCAAACACGTGGCACAGATTCTGGACTCC CGGATGAACACTAAGTACGACGAGAACGACAAACTGATCCGGGAAGTGAAAGTCATCACCCTGAAGTCCA AGCTGGTGTCTGATTTCCGGAAGGATTTCCAGTTCTACAAAGTGCGCGAGATCAACAACTACCATCACGC CCACGACGCCTACCTGAATGCCGTTGTTGGAACAGCCCTGATCAAGAAGTATCCCAAGCTGGAAAGCGAG TTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAAGAGATTGGCA AGGCTACCGCCAAGTACTTTTTCTACTCCAACATCATGAACTTTTTCAAGACAGAGATCACCCTGGCCAA CGGCGAGATCCGGAAAAGACCCCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGC AGAGATTTTGCCACAGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAGAAAACCGAGGTGC AGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCTAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAA GGACTGGGACCCAAAGAAGTACGGCGGCTTCGATAGCCCTACCGTGGCCTATTCTGTGCTGGTGGTGGCC AAAGTGGAAAAGGGCAAGTCCAAGAAACTCAAGAGCGTGAAAGAACTGCTCGGCATCACCATCATGGAAC GGTCCAGCTTTGAGAAGAACCCTATTGATTTCCTTGAGGCCAAGGGCTACAAAGAAGTGAAAAAGGACCT GATCATCAAGCTCCCCAAGTACTCCCTGTTCGAGCTGGAAAATGGCCGGAAGCGGATGCTGGCTTCTGCT GGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCTAGCAAATATGTGAACTTTCTGTACCTGGCCAGCC ACTATGAGAAGCTGAAGGGCAGCCCCGAGGACAATGAGCAAAAGCAGCTGTTTGTCGAGCAGCACAAGCA CTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCTGACGCCAATCTG GACAAAGTGCTGTCCGCCTACAACAAGCACCGGGACAAGCCTATCAGAGAGCAGGCCGAGAATATCATCC ACCTGTTTACCCTGACCAACCTGGGAGCCCCTGCCGCCTTCAAGTACTTCGACACCACCATCGACCGGAA GCGCTACACCAGCACCAAAGAGGTTCTGGACGCCACACTGATCCACCAGAGCATCACCGGCCTGTACGAG ACAAGAATCGACCTGTCTCAGCTCGGCGGCGATCCTCGAGGCTATCCCTATGACGTGCCCGATTATGCCA GCAGTGGCAGCGGCTCCCCCAAGAAAAAACGCAAGGTGGAAGATCCTAAGAAAAAGCGGAAAGTGGTTAA CGGAAGTGGCGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCACCTGCC GCTAAACGGGTCAAACTCGACGGCGGTTCTGGAGGTCCTGCTGCAAAGCGCGTTAAGTTGGATGCTAGCA TGGAATTTGTGTCTAAAGGGGAAGCAGTCATTAAAGAGTTCATGCGCTTCAAAGTACACATGGAAGGAAG TATGAACGGGCACGAATTTGAAATAGAAGGCGAAGGGGAAGGTCGGCCATACGAAGGGACGCAAACCGCG AAGTTGAAGGTTACAAAGGGAGGACCGTTGCCCTTTAGCTGGGACATCCTGTCTCCACAGTTTATGTACG GTTCAAGAGCTTTTACTAAGCACCCCGCTGACATCCCGGACTACTATAAACAGTCATTCCCCGAAGGATT CAAATGGGAGAGGGTCATGAATTTCGAGGACGGAGGTGCTGTTACAGTTACGCAGGATACCTCACTTGAG GATGGCACGCTTATATATAAGGTGAAGCTCCGCGGTACGAACTTTCCGCCGGACGGCCCCGTTATGCAGA AGAAGACGATGGGCTGGGAAGCCTCTACGGAACGCCTGTATCCTGAGGACGGTGTTCTGAAAGGGGATAT TAAAATGGCCCTTCGGTTGAAAGATGGGGGCCGCTATCTTGCGGACTTTAAGACCACCTACAAAGCAAAA AAGCCTGTCCAAATGCCCGGTGCATATAACGTCGATAGAAAACTTGATATAACCTCCCATAATGAAGATT ACACAGTAGTCGAGCAGTACGAACGAAGCGAAGGTCGGCACTCAACTGGAGGTATGGATGAATTGTATAA GTAGGATATCATGCCTAATTTTGAGTGTATTACTTTCGAAAATCAACCTCTGGATTACAAAATTTGTGAA AGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGT ATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTA TGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACT GGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGG CGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGT GGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGG ACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTC TGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCA TCGATACCGAGCGCTGGTCGACAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGG CCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGA CTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACA ACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAA TCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCAT GACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCC AACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTG CTCCCTTCCCTGTCCTTGTTTAAACGCGATCGCTTAATTAAGGCCGTGGAGGCCTTTTCCCCGTATCCCC CCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCG TGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAGCCCCG GGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACGTAATTACATCCCTGGGGGCTTTGGGGGGGGGCTG TCCCTCATAAAAGTTTTGTTACTTTATAGAAGAAATTTTGAGTTTTTGTTTTTTTTAATAAATAAATAAA CATAAATAAATTGTTTGTTGAATTTATTATTAGTATGTAAGTGTAAATATAATAAAACTTAATATCTATT CAAATTAATAAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAATTTTACACATGATTATCTT TAACGTACGTCACAATATGATTATCTTTCTAGGGTTAATCTAGCTGCGTGTTCTGCAGCGTGTCGAGCAT CTTCATCTGCTCCATCACGCTGTAAAACACATTTGCACCGCGAGTCTGCCCGTCCTCCACGGGTTCAAAA ACGTGAATGAACGAGGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTA CCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGA TCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCG GCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTT TCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGG GTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGT TCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTC GGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTT ACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTC AAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACC CAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGA TCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAA GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACT ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAG AGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGA GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAAC CGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTT GCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAG CCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGT TATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA CTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATT TTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGC GTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTAC CAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCC GTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCA GTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACT GAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCG GTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATA GTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCT ATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCC GCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCC TCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGT GAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGG CTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC CAAGCGCGCCCGCCGGGTAACTCACGGGGTATCCATGTCCATTTCTGCGGCATCCAGCCAGGATACCCGT CCTCGCTGACGTAATATCCCAGCGCCGCACCGCTGTCATTAATCTGCACACCGGCACGGCAGTTCCGGCT GTCGCCGGTATTGTTCGGGTTGCTGATGCGCTTCGGGCTGACCATCCGGAACTGTGTCCGGAAAAGCCGC GACGAACTGGTATCCCAGGTGGCCTGAACGAACAGTTCACCGTTAAAGGCGTGCATGGCCACACCTTCCC GAATCATCATGGTAAACGTGCGTTTTCGCTCAACGTCAATGCAGCAGCAGTCATCCTCGGCAAACTCTTT CCATGCCGCTTCAACCTCGCGGGAAAAGGCACGGGCTTCTTCCTCCCCGATGCCCAGATAGCGCCAGCTT GGGCGATGACTGAGCCGGAAAAAAGACCCGACGATATGATCCTGATGCAGCTAGATTAACCCTAGAAAGA TAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTC GCTGTGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTG TCACTGATTTTGAACTATAACGACCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAA GATTTAACTCATACGATAATTATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTATATATATAT TTTCTTGTTATAGAATCAGGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGC TAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCA GCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCT CTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAAGCCATACCAAATAACTTCGTATAATGTATGC TATACGAAGTTATCCATAGAGCCCACCGCATCCCCAGCATGCCTGCTATTGTCTTCCCAATCCTCCCCCT TGCTGTCCTGCCCCACCCCACCCCCCAGAATAGAATGACACCTACTCAGACAATGCGATGCAATTTCCTC ATTTTATTAGGAAAGGACAGTGGGAGTGGCACCTTCCAGGGTCAAGGAAGGCACGGGGGAGGGGCAAACA ACAGATGGCTGGCAACTAGAAGGCACAGAATTCTTAGCGGCCGCCTAATTAAGCTTGTGCCCCAGTTTGC TAGGGAGGTCGCAGTATCTGGCCACTGCCACCTCGTGCTGCTCGACGTAGGTCTCGTTGTTGGCCTCCTT GATTCTTTCCAGTCTGTAGTCCACATAGTAGACGCCAGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGAT CTATATGTGGTCTTGGCGTTTGCGATCAGATGGCTCCCGCCCACGAGCTTCAGGGCCATGTCGTTTCTGC CTTCCAGGCCGCCGTCAGCGGGGTACAGCGTCTCGGTGAAGGCCTCCCAGCCGAGTGTTTTCTTCTGCAT CACAGGGCCGTTGGATGTGAAGTTCACCCCTCTGATCTTGACGTTGTAGATGAGGCAGCCGTCCTGGAGG CTGGTGTCCTGGGTAGCGGTCAGCACGCCCCCGTCTTCGTATGTGGTGACTCTCTCCCATGTGAAGCCCT CAGGGAAGGACTGCTTGAAGAAGTCGGGGATGCCCTGGGTGTGGTTGATGAAGGTCTTGCTGCCGTAGAG GAAGCTAGTAGCCAGGATGTCGAAGGCGAAGGGGAGAGGGCCGCCCTCGACCACCTTGATTCTCATGGTC TGGGTGCCCTCGTAGGGCTTGCCTTCGCCCTCGGATGTGCACTTGAAGTGATGGTTGTCCACGGTGCCCT CCATGTACAGCTTCATGTGCATGTTCTCCTTAATCAGCTCTTCGCCCTTAGACACCATGGTGGCGGTGGC TCCCCCTGACCCTCCATCCAACTTAACGCGCTTTGCAGCAGGACCTCCAGAACCGCCGTCGAGTTTGACC CGTTTAGCGGCAGGGACCGGTGGGCCAGGATTCTCCTCGACGTCACCGCATGTTAGCAGACTTCCTCTGC CCTCTCCCGATCCGGCACCGGGCTTGCGGGTCATGCACCAGGTGCGCGGTCCTTCGGGCACCTCGACGTC GGCGGTGACGGTGAAGCCGAGCCGCTCGTAGAAGGGGAGGTTGCGGGGCGCGGAGGTCTCCAGGAAGGCG GGCACCCCGGCGCGCTCGGCCGCCTCCACTCCGGGGAGCACGACGGCGCTGCCCAGACCCTTGCCCTGGT GGTCGGGCGAGACTCCGACGGTGGCCAGGAACCACGCGGGCTCCTTGGGCCGGTGCGGCGCCAGGAGGCC TTCCATCTGTTGCTGCGCGGCCAGCCGGGAACCGCTCAACTCGGCCATGCGCGGGCCGATCTCGGCGAAC ACCGCCCCCGCTTCGACGCTCTCCGGCGTGGTCCAGACCGCCACGGCGGCGCCGTCGTCCGCGACCCACA CCTTGCCGATGTCGAGCCCGACGCGCGTGAGGAAGAGTTCTTGCAGCTCGGTGACCCGCTCGATGTGGCG GTCCGGATCGACGGTGTGGCGCGTGGCGGGGTAGTCGGCGAACGCGGCGGCGAGGGTGCGTACGGCCCTG GGGACGTCGTCGCGGGTGGCGAGGCGCACCGTGGGCTTGTACTCGGTCATGGTGGGATTTAAATCACGAC ACCTGAAATGGAAGAAAAAAACTTTGAACCACTGTCTGAGGCTTGAGAATGAACCAAGATCCAAACTCAA AAAGGGCAAATTCCAAGGAGAATTACATCAAGTGCCAAGCTGGCCTAACTTCAGTCTCCACCCACTCAGT GTGGGGAAACTCCATCGCATAAAACCCCTCCCCCCAACCTAAAGACGACGTACTCCAAAAGCGCGAGAAC TAATCGAGGTGCCTGGACGGCGCCCGGTACTCCGTGGAGTCACATGAAGCGACGGCTGAGGACGGAAAGG CCCTTTTCCTTTGTGTGGGTGACTCACCCGCCCGCTCTCCCGAGCGCCGCGTCCTCCATTTTGAGCTCCC TGCAGCAGGGCCGGGAAGCGGCCATCTTTCCGCTCACGCAACTGGTGCCGACCGGGCCAGCCTTGCCGCC CAGGGCGGGGCGATACACGGCGGCGCGAGGCCAGGCACCAGAGCAGGCCGGCCAGCTTGAGACTACCCCC GTCCGATTCTCGGTGGCCGCGCTCGCAGGCCCCGCCTCGCCGAACATGTGCGCTGGGACGCACGGGCCCC GTCGCCGCCCGCGGCCCCAAAAACCGAAATACCAGTGTGCAGATCTTGGCCCGCATTTACAAGACTATCT TGCCAGAAAAAAAGCGTCGCAGCAGGTCATCAAAAATTTTAAATGGCTAGAGACTTATCGAAAGCAGCGA GACAGGCGCGAAGGTGCCACCAGATTCGCACGCGGCGGCCCCAGCGCCCAGGCCAGGCCTCAACTCAAGC ACGAGGCGAAGGGGCTCCTTAAGCGCAAGGCCTCGAACTCTCCCACCCACTTCCAACCCGAAGCTCGGGA TCAAGAATCACGTACTGCAGCCAGGTGGAAGTAATTCAAGGCACGCAAGGGCCATAACCCGTAAAGAGGC CAGGCCCGCGGGAACCACACACGGCACTTACCTGTGTTCTGGCGGCAAACCCGTTGCGAAAAAGAACGTT CACGGCGACTACTGCACTTATATACGGTTCTCCCCCACCCTCGGGAAAAAGGCGGAGCCAGTACACGACA TCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTAGGCACCGGTTCAATTGCCGACCCCTCCCCCCAACT TCTCGGGGACTGTGGGCGATGTGCGCTCTGCCCATAACTTCGTATAATGTATGCTATACGAAGTTATAAT ATTCTCACTGACTCCGTCCTGGAGTTGGATGAGAGATAATGGCCTTACGTTGTGCCAGGGGAGGGTCGGG CTGGATTTAGCAAGATTTACCTTCTCCAAAGAGCGGTGCTGCAGTGGCACAGCTGCCCACGGAGGTGGGG GGGTCACCGTCCCTGGAGGTGATGAAGAACTGTGGGGATGTGGCACTGAGGGACATGGCCAGTGGGCACG GTGGGTGGGTTGGGGTTGGTCTTGGGGATCTTGGAGGGCTTTTCCAGCCTTCATGATTTGACGATTGTAT GAACATCTACATGGCAATTCTCCAGCTGCCTGTCCCAGTCCTACTGACCCAGCTGTATCTCTCCAGGCAA GCTCTTCCACCCCTTCTGCTTGCATCCAGACACCATCAAACATGCAGGCTCAGACACAGGGACCAGCAGT GTCTGTGGCCTTTTTGTGCTCCTCTCCATGCTGGGTTTTAACTTGCTCTTTGTCCTTCTATCCTATCTTC TTATCCTTAAGGCTGTTCTGAACGCTGTGACTTGGAGAGTGTCCCAGAGCCCTCAACACCTGCATGTCCC ACGTCCATGCTGTCCTGCACTTCCTTATCCCCAAGATCTGCCTCTCCGTGATGCACTGAATTGGCAAACA TGTGTCACCCCAGACCAACAATGTCACAGCAAACTCCCCCTTGATAGGACAAGGGGGAATGGCTTTACAC TGAGACAGGGGAGGTTTGGGTTGGATATGAGGAGGCAGTTTTTCCCCCAGAGGGTGGTGACGCACTGAAC AGGTTGCCCAAGGAGGCTGTGGATGCCCCATCCCTGCAGGCATTCAAGGCCAGGCTGGATGTGGCTCTGG GCAGCCTGGGCTGCTGGTTGATGACCCTGCACATAGCAGGGGGTTGGATCTGGATGAGCACTGTGCTCCT TTGCAACCCAGGCCGTTCTATGATTCTGTCATTCTAAATCTCTCTTTCAGCCTAAAGCTTTTTCCCCGTA TCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTT CCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAG CCCCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACGTAATTACATCCCTGGGGGCTTTGGGGGGG GGCTGTCCCCGTGAGCTCCACGTGCCTAGGGCACACGACCACAATTCCACTGAAAGCATTTTAATACGGA ACTTGTCACTCCCAGGGAGCCTCCGCTCAGCCGGCAGTTGGTTCATTTCAATCCCCACGACAACCCTTCA AAGTGCAGGGCAGACAGCAGGTGGCTCTGCCCAGGCGCCTGGATCACAGCCCGGCCTGCAGCCCTCACCT GGGCGCGGGGAGACCCTGAGGACGCTCCTCCAGGCGGCGCTGGCCGGGGCCTGCGGACACGGACGGGCGG GCTGAGCTCCGGGACCCCTCCCCGCGCCCCGCACCCCGCACCCCGCACCCCGCACCCCGCACCCGGCGCT CACCCGTCCCAGCCCCGCCGCCCGCAGCCCCAGCTGCAACGCAGCCACCGCCGCCATCGCACCCGGCCCC GCGGGCGCTTCCGGGACGCAGGAGGCATCTGCATCCGGGGCGCCGCTGAGTCCCGCCCAGAGCCCCGCCC CCGGCTCCAGGTTCTGCGAGCGGCTTCCGCCGGGCTGCTCCGCGGGCGCGTCGGCCATGAGCGAGTTGCC GGGCGACGTGCGGGCGTTTCTGCGGGAGCACCCGAGCCTGCGGCTCCAGACGGACGCCCGCAAGGTTCGC AGCGCGGGAGGGGAACGGAGTGGCGGAGAAGGGCGCAGTTGGGATGAGGGGCTGAGGGGAGGGCAGGGGA GAGGAGAGGGCAGGGGAGAGGGGAGAGGGGAGAGCAGGAGAGAGGGGAAGGCAGGGGAGAGGGCGCGGCG GGATCAGGGGAGGAGAGGGAAGGGGGCGCGGCAGGAGGGGGCACCAGGGAGCGGAGCCCTGGCCCTCCTG ACGTCCTGCCCGCCCACGCGTCCGCAGGTGAGGTGCATCCTGACAGGTCACGAGCTGCCCTGCCGCCTGC CGGAGCTCCAGGTCTACACCCGCGGCAAAAAGTACCAGCGGCTGGTCCGCGCCTCCCCGGCCTTCGACTA TGCAGAGTTCGAGCCGCACATCGTGCCCAGCACCAAGAACCCGTAGGTGGTC

iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP Sequences (1)

Full Sequences from Addgene (1)

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