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iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
(Plasmid #204729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    M54
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zim3-dCas9
  • Alt name
    PB-CAG(LoxStopLox-Zim3-XTENsion-dCas9-HA-2(svNLS)-T2A-2(mycNLS)-mScarlet-WPRE-pA)-EF1a(PuroR-T2A-BFP-pA)
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP was a gift from iPSC Neurodegenerative Disease Initiative (iNDI) & Michael Ward (Addgene plasmid # 204729 ; http://n2t.net/addgene:204729 ; RRID:Addgene_204729)
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