iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

Analyze Sequence

GenBank

SnapGene

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> Addgene Partial NGS Result ATTAAGGCCGTGGAGGCCTTTTCCCCGTATCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCG TTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGA TGCGGGGGGAGCGCCGGACCGGAGCGGAGCCCCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACG TAATTACATCCCTGGGGGCTTTGGGGGGGGGCTGTCCCTCATAAAAGTTTTGTTACTTTATAGAAGAAAT TTTGAGTTTTTGTTTTTTTTAATAAATAAATAAACATAAATAAATTGTTTGTTGAATTTATTATTAGTAT GTAAGTGTAAATATAATAAAACTTAATATCTATTCAAATTAATAAATAAACCTCGATATACAGACCGATA AAACACATGCGTCAATTTTACACATGATTATCTTTAACGTACGTCACAATATGATTATCTTTCTAGGGTT AATCTAGCTGCGTGTTCTGCAGCGTGTCGAGCATCTTCATCTGCTCCATCACGCTGTAAAACACATTTGC ACCGCGAGTCTGCCCGTCCTCCACGGGTTCAAAAACGTGAATGAACGAGGCGCGCTCACTGGCCGTCGTT TTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCG CCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGA ATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACA CTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTC CCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAA AAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACG TTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCT ATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAA ATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCG GAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATA AATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATC AGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCC CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCA CAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAA CACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTG ACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGC TTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCAC TGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTG ATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGAT CAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTA CCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAG CGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACC GGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACAC AGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCAC GCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGG GAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTC GATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTT CCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGT ATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCG AGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTG GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCAT TAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAAT TTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCCCGCCGGGTAACTCACGGGGTATCCAT GTCCATTTCTGCGGCATCCAGCCAGGATACCCGTCCTCGCTGACGTAATATCCCAGCGCCGCACCGCTGT CATTAATCTGCACACCGGCACGGCAGTTCCGGCTGTCGCCGGTATTGTTCGGGTTGCTGATGCGCTTCGG GCTGACCATCCGGAACTGTGTCCGGAAAAGCCGCGACGAACTGGTATCCCAGGTGGCCTGAACGAACAGT TCACCGTTAAAGGCGTGCATGGCCACACCTTCCCGAATCATCATGGTAAACGTGCGTTTTCGCTCAACGT CAATGCAGCAGCAGTCATCCTCGGCAAACTCTTTCCATGCCGCTTCAACCTCGCGGGAAAAGGCACGGGC TTCTTCCTCCCCGATGCCCAGATAGCGCCAGCTTGGGCGATGACTGAGCCGGAAAAAAGACCCGACGATA TGATCCTGATGCAGCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT ATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGGACATCTCAGTCGCCGCTTGGAG CTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTGATTTTGAACTATAACGACCGCGTGAGTCAA AATGACGCATGATTATCTTTTACGTGACTTTTAAGATTTAACTCATACGATAATTATATTGTTATTTCAT GTTCTACTTACGTGATAACTTATTATATATATATTTTCTTGTTATAGAATCAGGGACAGCCCCCCCCCAA AGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCC GGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCA CGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAA AAAGCCATACCAAATAACTTCGTATAATGTATGCTATACGAAGTTATCCATAGAGCCCACCGCATCCCCA GCATGCCTGCTATTGTCTTCCCAATCCTCCCCCTTGCTGTCCTGCCCCACCCCACCCCCCAGAATAGAAT GACACCTACTCAGACAATGCGATGCAATTTCCTCATTTTATTAGGAAAGGACAGTGGGAGTGGCACCTTC CAGGGTCAAGGAAGGCACGGGGGAGGGGCAAACAACAGATGGCTGGCAACTAGAAGGCACAGAATTCTTA GCGGCCGCCTAATTAAGCTTGTGCCCCAGTTTGCTAGGGAGGTCGCAGTATCTGGCCACTGCCACCTCGT GCTGCTCGACGTAGGTCTCGTTGTTGGCCTCCTTGATTCTTTCCAGTCTGTAGTCCACATAGTAGACGCC AGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGATCTATATGTGGTCTTGGCGTTTGCGATCAGATGGCTC CCGCCCACGAGCTTCAGGGCCATGTCGTTTCTGCCTTCCAGGCCGCCGTCAGCGGGGTACAGCGTCTCGG TGAAGGCCTCCCAGCCGAGTGTTTTCTTCTGCATCACAGGGCCGTTGGATGTGAAGTTCACCCCTCTGAT CTTGACGTTGTAGATGAGGCAGCCGTCCTGGAGGCTGGTGTCCTGGGTAGCGGTCAGCACGCCCCCGTCT TCGTATGTGGTGACTCTCTCCCATGTGAAGCCCTCAGGGAAGGACTGCTTGAAGAAGTCGGGGATGCCCT GGGTGTGGTTGATGAAGGTCTTGCTGCCGTAGAGGAAGCTAGTAGCCAGGATGTCGAAGGCGAAGGGGAG AGGGCCGCCCTCGACCACCTTGATTCTCATGGTCTGGGTGCCCTCGTAGGGCTTGCCTTCGCCCTCGGAT GTGCACTTGAAGTGATGGTTGTCCACGGTGCCCTCCATGTACAGCTTCATGTGCATGTTCTCCTTAATCA GCTCTTCGCCCTTAGACACCATGGTGGCGGTGGCTCCCCCTGACCCTCCATCCAACTTAACGCGCTTTGC AGCAGGACCTCCAGAACCGCCGTCGAGTTTGACCCGTTTAGCGGCAGGGACCGGTGGGCCAGGATTCTCC TCGACGTCACCGCATGTTAGCAGACTTCCTCTGCCCTCTCCCGATCCGGCACCGGGCTTGCGGGTCATGC ACCAGGTGCGCGGTCCTTCGGGCACCTCGACGTCGGCGGTGACGGTGAAGCCGAGCCGCTCGTAGAAGGG GAGGTTGCGGGGCGCGGAGGTCTCCAGGAAGGCGGGCACCCCGGCGCGCTCGGCCGCCTCCACTCCGGGG AGCACGACGGCGCTGCCCAGACCCTTGCCCTGGTGGTCGGGCGAGACTCCGACGGTGGCCAGGAACCACG CGGGCTCCTTGGGCCGGTGCGGCGCCAGGAGGCCTTCCATCTGTTGCTGCGCGGCCAGCCGGGAACCGCT CAACTCGGCCATGCGCGGGCCGATCTCGGCGAACACCGCCCCCGCTTCGACGCTCTCCGGCGTGGTCCAG ACCGCCACGGCGGCGCCGTCGTCCGCGACCCACACCTTGCCGATGTCGAGCCCGACGCGCGTGAGGAAGA GTTCTTGCAGCTCGGTGACCCGCTCGATGTGGCGGTCCGGATCGACGGTGTGGCGCGTGGCGGGGTAGTC GGCGAACGCGGCGGCGAGGGTGCGTACGGCCCTGGGGACGTCGTCGCGGGTGGCGAGGCGCACCGTGGGC TTGTACTCGGTCATGGTGGGATTTAAATCACGACACCTGAAATGGAAGAAAAAAACTTTGAACCACTGTC TGAGGCTTGAGAATGAACCAAGATCCAAACTCAAAAAGGGCAAATTCCAAGGAGAATTACATCAAGTGCC AAGCTGGCCTAACTTCAGTCTCCACCCACTCAGTGTGGGGAAACTCCATCGCATAAAACCCCTCCCCCCA ACCTAAAGACGACGTACTCCAAAAGCGCGAGAACTAATCGAGGTGCCTGGACGGCGCCCGGTACTCCGTG GAGTCACATGAAGCGACGGCTGAGGACGGAAAGGCCCTTTTCCTTTGTGTGGGTGACTCACCCGCCCGCT CTCCCGAGCGCCGCGTCCTCCATTTTGAGCTCCCTGCAGCAGGGCCGGGAAGCGGCCATCTTTCCGCTCA CGCAACTGGTGCCGACCGGGCCAGCCTTGCCGCCCAGGGCGGGGCGATACACGGCGGCGCGAGGCCAGGC ACCAGAGCAGGCCGGCCAGCTTGAGACTACCCCCGTCCGATTCTCGGTGGCCGCGCTCGCAGGCCCCGCC TCGCCGAACATGTGCGCTGGGACGCACGGGCCCCGTCGCCGCCCGCGGCCCCAAAAACCGAAATACCAGT GTGCAGATCTTGGCCCGCATTTACAAGACTATCTTGCCAGAAAAAAAGCGTCGCAGCAGGTCATCAAAAA TTTTAAATGGCTAGAGACTTATCGAAAGCAGCGAGACAGGCGCGAAGGTGCCACCAGATTCGCACGCGGC GGCCCCAGCGCCCAGGCCAGGCCTCAACTCAAGCACGAGGCGAAGGGGCTCCTTAAGCGCAAGGCCTCGA ACTCTCCCACCCACTTCCAACCCGAAGCTCGGGATCAAGAATCACGTACTGCAGCCAGGTGGAAGTAATT CAAGGCACGCAAGGGCCATAACCCGTAAAGAGGCCAGGCCCGCGGGAACCACACACGGCACTTACCTGTG TTCTGGCGGCAAACCCGTTGCGAAAAAGAACGTTCACGGCGACTACTGCACTTATATACGGTTCTCCCCC ACCCTCGGGAAAAAGGCGGAGCCAGTACACGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTAG GCACCGGTTCAATTGCCGACCCCTCCCCCCAACTTCTCGGGGACTGTGGGCGATGTGCGCTCTGCCCATA ACTTCGTATAATGTATGCTATACGAAGTTATAATATTCTCACTGACTCCGTCCTGGAGTTGGATGAGAGA TAATGGCCTTACGTTGTGCCAGGGGAGGGTCGGGCTGGATTTAGCAAGATTTACCTTCTCCAAAGAGCGG TGCTGCAGTGGCACAGCTGCCCACGGAGGTGGGGGGGTCACCGTCCCTGGAGGTGATGAAGAACTGTGGG GATGTGGCACTGAGGGACATGGCCAGTGGGCACGGTGGGTGGGTTGGGGTTGGTCTTGGGGATCTTGGAG GGCTTTTCCAGCCTTCATGATTTGACGATTGTATGAACATCTACATGGCAATTCTCCAGCTGCCTGTCCC AGTCCTACTGACCCAGCTGTATCTCTCCAGGCAAGCTCTTCCACCCCTTCTGCTTGCATCCAGACACCAT CAAACATGCAGGCTCAGACACAGGGACCAGCAGTGTCTGTGGCCTTTTTGTGCTCCTCTCCATGCTGGGT TTTAACTTGCTCTTTGTCCTTCTATCCTATCTTCTTATCCTTAAGGCTGTTCTGAACGCTGTGACTTGGA GAGTGTCCCAGAGCCCTCAACACCTGCATGTCCCACGTCCATGCTGTCCTGCACTTCCTTATCCCCAAGA TCTGCCTCTCCGTGATGCACTGAATTGGCAAACATGTGTCACCCCAGACCAACAATGTCACAGCAAACTC CCCCTTGATAGGACAAGGGGGAATGGCTTTACACTGAGACAGGGGAGGTTTGGGTTGGATATGAGGAGGC AGTTTTTCCCCCAGAGGGTGGTGACGCACTGAACAGGTTGCCCAAGGAGGCTGTGGATGCCCCATCCCTG CAGGCATTCAAGGCCAGGCTGGATGTGGCTCTGGGCAGCCTGGGCTGCTGGTTGATGACCCTGCACATAG CAGGGGGTTGGATCTGGATGAGCACTGTGCTCCTTTGCAACCCAGGCCGTTCTATGATTCTGTCATTCTA AATCTCTCTTTCAGCCTAAAGCTTTTTCCCCGTATCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAG AAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTC GGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAGCCCCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAG GGACGTAATTACATCCCTGGGGGCTTTGGGGGGGGGCTGTCCCCGTGAGCTCCACGTGCCTAGGGCACAC GACCACAATTCCACTGAAAGCATTTTAATACGGAACTTGTCACTCCCAGGGAGCCTCCGCTCAGCCGGCA GTTGGTTCATTTCAATCCCCACGACAACCCTTCAAAGTGCAGGGCAGACAGCAGGTGGCTCTGCCCAGGC GCCTGGATCACAGCCCGGCCTGCAGCCCTCACCTGGGCGCGGGGAGACCCTGAGGACGCTCCTCCAGGCG GCGCTGGCCGGGGCCTGCGGACACGGACGGGCGGGCTGAGCTCCGGGACCCCTCCCCGCGCCCCGCACCC CGCACCCCGCACCCCGCACCCCGCACCCGGCGCTCACCCGTCCCAGCCCCGCCGCCCGCAGCCCCAGCTG CAACGCAGCCACCGCCGCCATCGCACCCGGCCCCGCGGGCGCTTCCGGGACGCAGGAGGCATCTGCATCC GGGGCGCCGCTGAGTCCCGCCCAGAGCCCCGCCCCCGGCTCCAGGTTCTGCGAGCGGCTTCCGCCGGGCT GCTCCGCGGGCGCGTCGGCCATGAGCGAGTTGCCGGGCGACGTGCGGGCGTTTCTGCGGGAGCACCCGAG CCTGCGGCTCCAGACGGACGCCCGCAAGGTTCGCAGCGCGGGAGGGGAACGGAGTGGCGGAGAAGGGCGC AGTTGGGATGAGGGGCTGAGGGGAGGGCAGGGGAGAGGAGAGGGCAGGGGAGAGGGGAGAGGGGAGAGCA GGAGAGAGGGGAAGGCAGGGGAGAGGGCGCGGCGGGATCAGGGGAGGAGAGGGAAGGGGGCGCGGCAGGA GGGGGCACCAGGGAGCGGAGCCCTGGCCCTCCTGACGTCCTGCCCGCCCACGCGTCCGCAGGTGAGGTGC ATCCTGACAGGTCACGAGCTGCCCTGCCGCCTGCCGGAGCTCCAGGTCTACACCCGCGGCAAAAAGTACC AGCGGCTGGTCCGCGCCTCCCCGGCCTTCGACTATGCAGAGTTCGAGCCGCACATCGTGCCCAGCACCAA GAACCCGTAGGTGGTCGGCGCGCCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTC ATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCC AAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTA TGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCA CGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATT ATTTTGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGGC GGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTAT GGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTGC CTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCC CACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGT TTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCG GGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGC GCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGC CCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCA GGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCC CGGCTTCGGGTGCGGGGCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCC CGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGG CGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAG CGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCG CGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGAC GGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGC CTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA TGGTACCGTTAACGCGATCGCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCCCGCGATGAATAAAT GAAAGCTTGCAGATCTGCGACCCTAGAGGATCTGCGACCCTAGAGGATCATAATCAGCCATACCACATTT GTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAA TTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCAC AAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTC TGGATCTGCGACCCTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAA CCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCA GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATT CTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGCGACCCTAGAGGATCATA ATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGA AACTAAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAA TAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATC AATGTATCTTATCATGTCTGGATCTCCATCAAGCTGATCCGGAACCCTTAATGAAGTTCCTATTCTCTAG AAAGTATAGGAACTTCGTATACTCGCGAGCCGCCACCATGAACAACTCTCAGGGCAGAGTGACCTTCGAG GACGTGACCGTGAACTTCACCCAAGGCGAGTGGCAGAGACTGAACCCCGAGCAGAGAAACCTGTACCGGG ACGTGATGCTGGAAAACTACAGCAACCTGGTGTCCGTCGGCCAGGGCGAGACAACAAAGCCTGACGTGAT CCTGCGGCTGGAACAGGGCAAAGAGCCTTGGCTGGAAGAGGAAGAGGTGCTCGGATCTGGCAGAGCCGAG AAGAATGGCGACATCGGCGGACAGATCTGGAAGCCCAAGGACGTGAAAGAGTCTCTGAGTGGCGGCTCAA GTGGCGGGTCTAGTGGGAGTGAAACGCCAGGGACTTCAGAATCAGCAACCCCGGAATCATCCGGCGGATC ATCCGGTGGGTCTGTATACATGGACAAGAAGTACTCTATCGGCCTGGCCATCGGCACCAATTCTGTTGGA TGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGC ACAGCATCAAGAAGAATCTGATCGGCGCCCTGCTGTTCGACTCTGGCGAAACAGCCGAAGCCACCAGACT GAAGAGAACCGCCAGACGGCGGTACACCAGAAGAAAGAACCGGATCTGCTACCTGCAAGAGATCTTCAGC AACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTCGAGGAAGATA AGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGATGAGGTGGCCTACCACGAGAAGTACCCTAC CATCTACCACCTCCGGAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGAGACTGATCTATCTGGCC CTGGCTCACATGATCAAGTTCCGGGGCCACTTTCTGATCGAGGGCGACCTGAATCCTGACAACAGCGACG TGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAG CGGAGTGGATGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGCCTGGAAAACCTGATCGCT CAGCTGCCCGGCGAGAAGAAGAACGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACACCTA ACTTCAAGAGCAACTTCGACCTGGCTGAGGACGCCAAACTGCAGCTGTCCAAGGACACCTACGACGACGA CCTGGACAATCTGCTGGCCCAGATCGGCGATCAGTACGCCGACTTGTTTCTGGCCGCCAAGAACCTGTCC GACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACAAAGGCCCCTCTGAGCGCCTCTA TGATCAAGAGATACGACGAGCACCACCAGGATCTGACCCTGCTGAAGGCCCTCGTTAGACAGCAGCTGCC TGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGATGGCGGAGCC AGCCAAGAGGAATTTTACAAGTTCATCAAGCCCATCCTTGAGAAGATGGACGGCACCGAGGAACTGCTGG TCAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAATGGCTCTATCCCTCACCAGAT CCACCTGGGAGAGCTGCACGCCATTCTGCGGAGACAAGAGGACTTTTACCCATTCCTGAAGGACAACCGG GAAAAGATTGAGAAGATCCTGACCTTCAGAATCCCCTACTACGTGGGACCACTGGCCAGAGGCAATAGCA GATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACACCCTGGAACTTCGAGGAAGTGGTGGACAA GGGCGCCAGCGCTCAGTCCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCTAACGAGAAGGTG CTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGA CCGAGGGAATGAGAAAGCCCGCCTTTCTGAGCGGCGAGCAGAAAAAGGCCATTGTGGATCTGCTGTTCAA GACCAACCGGAAAGTGACAGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACAGC GTGGAAATCAGCGGCGTGGAAGATCGGTTCAATGCCAGCCTGGGCACATACCACGACCTGCTGAAAATTA TCAAGGACAAGGACTTCCTGGACAACGAAGAGAACGAGGACATCCTGGAAGACATCGTGCTGACCCTGAC ACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACATACGCCCACCTGTTCGACGACAAAGTG ATGAAGCAACTGAAGCGGCGGAGATACACCGGCTGGGGTAGACTGTCCCGGAAGCTGATCAACGGCATCC GGGATAAGCAGTCCGGCAAGACCATCCTGGACTTTCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCAT GCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAAGGC GATTCTCTGCACGAGCACATTGCCAACCTGGCCGGATCTCCCGCCATTAAGAAGGGCATCCTGCAGACCG TGAAGGTGGTGGACGAGCTTGTGAAAGTGATGGGCAGACACAAGCCCGAGAACATCGTGATCGAAATGGC CAGAGAGAACCAGACCACACAGAAGGGCCAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGC ATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGC TGTACCTGTACTACCTGCAGAATGGACGGGATATGTACGTGGACCAAGAGCTGGACATCAACCGGCTGTC CGACTACGATGTGGACGCTATCGTGCCCCAGTCTTTTCTGAAGGACGACTCCATCGACAACAAGGTCCTG ACCAGAAGCGACAAGAACCGGGGCAAGAGCGATAACGTGCCCTCCGAAGAGGTGGTCAAGAAGATGAAGA ACTATTGGCGCCAGCTGCTGAATGCCAAGCTGATTACCCAGCGGAAGTTCGACAACCTGACCAAGGCCGA AAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATC ACCAAACACGTGGCACAGATTCTGGACTCCCGGATGAACACTAAGTACGACGAGAACGACAAACTGATCC GGGAAGTGAAAGTCATCACCCTGAAGTCCAAGCTGGTGTCTGATTTCCGGAAGGATTTCCAGTTCTACAA AGTGCGCGAGATCAACAACTACCATCACGCCCACGACGCCTACCTGAATGCCGTTGTTGGAACAGCCCTG ATCAAGAAGTATCCCAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGA TGATCGCCAAGAGCGAGCAAGAGATTGGCAAGGCTACCGCCAAGTACTTTTTCTACTCCAACATCATGAA CTTTTTCAAGACAGAGATCACCCTGGCCAACGGCGAGATCCGGAAAAGACCCCTGATCGAGACAAACGGC GAAACCGGGGAGATCGTGTGGGATAAGGGCAGAGATTTTGCCACAGTGCGGAAAGTGCTGAGCATGCCCC AAGTGAATATCGTGAAGAAAACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCTAAGCG GAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCAAAGAAGTACGGCGGCTTCGATAGCCCT ACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTCAAGAGCGTGA AAGAACTGCTCGGCATCACCATCATGGAACGGTCCAGCTTTGAGAAGAACCCTATTGATTTCCTTGAGGC CAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTCCCCAAGTACTCCCTGTTCGAGCTGGAA AATGGCCGGAAGCGGATGCTGGCTTCTGCTGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCTAGCA AATATGTGAACTTTCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCAGCCCCGAGGACAATGAGCA AAAGCAGCTGTTTGTCGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCC AAGAGAGTGATCCTGGCTGACGCCAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGACAAGC CTATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAACCTGGGAGCCCCTGCCGCCTT CAAGTACTTCGACACCACCATCGACCGGAAGCGCTACACCAGCACCAAAGAGGTTCTGGACGCCACACTG ATCCACCAGAGCATCACCGGCCTGTACGAGACAAGAATCGACCTGTCTCAGCTCGGCGGCGATCCTCGAG GCTATCCCTATGACGTGCCCGATTATGCCAGCAGTGGCAGCGGCTCCCCCAAGAAAAAACGCAAGGTGGA AGATCCTAAGAAAAAGCGGAAAGTGGTTAACGGAAGTGGCGAGGGCAGAGGAAGTCTGCTAACATGCGGT GACGTCGAGGAGAATCCTGGCCCACCTGCCGCTAAACGGGTCAAACTCGACGGCGGTTCTGGAGGTCCTG CTGCAAAGCGCGTTAAGTTGGATGCTAGCATGGAATTTGTGTCTAAAGGGGAAGCAGTCATTAAAGAGTT CATGCGCTTCAAAGTACACATGGAAGGAAGTATGAACGGGCACGAATTTGAAATAGAAGGCGAAGGGGAA GGTCGGCCATACGAAGGGACGCAAACCGCGAAGTTGAAGGTTACAAAGGGAGGACCGTTGCCCTTTAGCT GGGACATCCTGTCTCCACAGTTTATGTACGGTTCAAGAGCTTTTACTAAGCACCCCGCTGACATCCCGGA CTACTATAAACAGTCATTCCCCGAAGGATTCAAATGGGAGAGGGTCATGAATTTCGAGGACGGAGGTGCT GTTACAGTTACGCAGGATACCTCACTTGAGGATGGCACGCTTATATATAAGGTGAAGCTCCGCGGTACGA ACTTTCCGCCGGACGGCCCCGTTATGCAGAAGAAGACGATGGGCTGGGAAGCCTCTACGGAACGCCTGTA TCCTGAGGACGGTGTTCTGAAAGGGGATATTAAAATGGCCCTTCGGTTGAAAGATGGGGGCCGCTATCTT GCGGACTTTAAGACCACCTACAAAGCAAAAAAGCCTGTCCAAATGCCCGGTGCATATAACGTCGATAGAA AACTTGATATAACCTCCCATAATGAAGATTACACAGTAGTCGAGCAGTACGAACGAAGCGAAGGTCGGCA CTCAACTGGAGGTATGGATGAATTGTATAAGTAGGATATCATGCCTAATTTTGAGTGTATTACTTTCGAA AATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGC TATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTC CTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTG TGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGA CTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGG GGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTC GCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGG ACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAG TCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGAGCGCTGGTCGACAGATCTACGGGTGGCATC CCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCT AATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTG GTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGA GTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCC TCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGG GGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCA AATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTGTTTAAACGCGATCGCTTA

iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP Sequences (1)

Full Sequences from Addgene (1)

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