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pTRIP-hPGK-Blast-2A-STING-TurboID
(Plasmid #204713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIP
  • Backbone size w/o insert (bp) 9119
  • Total vector size (bp) 12237
  • Modifications to backbone
    Added hPGK promoter, blast resistance gene, 2A peptide
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STING1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1137
  • Entrez Gene
    STING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
  • Promoter hPGK
  • Tag / Fusion Protein
    • TurboID (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tgagaacaggggcatcttga
  • 3′ sequencing primer ctaagatctacagctgccttgt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP-hPGK-Blast-2A-STING-TurboID was a gift from Nir Hacohen (Addgene plasmid # 204713 ; http://n2t.net/addgene:204713 ; RRID:Addgene_204713)

  • For your References section:

    ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling. Gentili M, Liu B, Papanastasiou M, Dele-Oni D, Schwartz MA, Carlson RJ, Al'Khafaji AM, Krug K, Brown A, Doench JG, Carr SA, Hacohen N. Nat Commun. 2023 Feb 4;14(1):611. doi: 10.1038/s41467-023-36132-9. 10.1038/s41467-023-36132-9 PubMed 36739287
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