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Addgene

pMXSIP-GPR35-FLAG-APEX
(Plasmid #204706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein-coupled receptor 35
  • Species
    H. sapiens (human)
  • Entrez Gene
    GPR35
  • Tag / Fusion Protein
    • FLAG, APEX (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer TTGGATACACGCCGCCCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXSIP-GPR35-FLAG-APEX was a gift from William Kaelin (Addgene plasmid # 204706 ; http://n2t.net/addgene:204706 ; RRID:Addgene_204706)

  • For your References section:

    Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists. Wyant GA, Yu W, Doulamis IP, Nomoto RS, Saeed MY, Duignan T, McCully JD, Kaelin WG Jr. Science. 2022 Aug 5;377(6606):621-629. doi: 10.1126/science.abm1638. Epub 2022 Aug 4. 10.1126/science.abm1638 PubMed 35926043
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