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Addgene

p164-pAAV-mDlx-FLEX-mScarlet
(Plasmid #204689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-mDlx-dio
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 4915
  • Total vector size (bp) 5697
  • Modifications to backbone
    mScarlet fluorescent reporter added to the vector backbone
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet
  • Alt name
    mScarlet
  • Species
    Synthetic
  • Insert Size (bp)
    770
  • Promoter mDlx
  • Tag / Fusion Protein
    • mScarlet palmitoylation (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site FseI (unknown if destroyed)
  • 5′ sequencing primer TTACTTGTACAGCTCGTCCATGC
  • 3′ sequencing primer CCACCATGCTGTGCTGTATGAGAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

11bp sequence missing in AAV2 ITR sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p164-pAAV-mDlx-FLEX-mScarlet was a gift from Lynette Lim (Addgene plasmid # 204689 ; http://n2t.net/addgene:204689 ; RRID:Addgene_204689)

  • For your References section:

    Cortical somatostatin long-range projection neurons and interneurons exhibit divergent developmental trajectories. Fisher J, Verhagen M, Long Z, Moissidis M, Yan Y, He C, Wang J, Micoli E, Alastruey CM, Moors R, Marin O, Mi D, Lim L. Neuron. 2023 Dec 7:S0896-6273(23)00887-5. doi: 10.1016/j.neuron.2023.11.013. 10.1016/j.neuron.2023.11.013 PubMed 38086373
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